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By a modification of the Maximow method of tissue culture the white blood cells from a number of patients with leukemia were cultured. These cultures were observed in the living condition, also fixed at six hour intervals or less during the first 24 hours, and at 12 hour intervals or less after that time. After fixation in Zenker's solution plus formaldehyde, they were embedded in celloidin, serially sectioned and stained with a hematoxylin-eosin-azure II solution. Two patients with chronic myelogenous, one with chronic lymphatic, three with myeloblastic, and one with acute lymphatic, leukemia furnished the white blood cells upon the
Cultures of Leukemic Blood Leucocytes.. Ann Intern Med. ;6:587–588. doi: 10.7326/0003-4819-6-4-587_3
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Published: Ann Intern Med. 1932;6(4):587-588.
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Print ISSN: 0003-4819 | Online ISSN: 1539-3704
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