THOMAS R. ELDER, M.B., CH.B.; JACK L. BARRY, M.D.; PAULINE M. FOLCIK; EDWIN M. KNIGHTS JR., M.D., F.A.C.P.
Chemical tests for the detection of blood in the urine are based on the ability of hemoglobin to catalyze the oxidation of an indicator substance by hydrogen peroxide. In this investigation a new reagent stick test (Hemastix1) was evaluated. It consists of a stiff bibulous strip of cellulose in which the test end is impregnated with the reagents: o-tolidine as the indicator, cumene hydroperoxide in a stabilized form, and a citrate buffer to maintain the optimal pH. Hemoglobin catalyzes the oxidation of o-tolidine by cumene hydroperoxide to form a blue color. Also used in the investigation were commercially available tablets
ELDER TR, BARRY JL, FOLCIK PM, KNIGHTS EM. Simplified Test for Hematuria. Ann Intern Med. ;56:957–959. doi: 10.7326/0003-4819-56-6-957
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Published: Ann Intern Med. 1962;56(6):957-959.
Nephrology, Urological Disorders.
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