Robert H. Bigley, M.D.; Jose O. Campos, M.D.; Robert D. Koler, M.D., F.A.C.P.
This content is PDF only. Please click on the PDF icon to access.
Studies of purified leukocyte and red cell pyruvate kinase (PK) show different kinetics, suggesting that they are different enzymes with different mechanisms of action. In the forward reaction, the two substrates, adenosine diphosphate (ADP) and phosphoenolpyruate (PEP) combine with leukocyte PK in a noncompulsory or independent fashion with Km's very similar to those reported for rabbit muscle PK. Red cell enzyme kinetics, on the contrary, suggest compulsory combination with substrates. Since the affinity for one substrate is modified by the concentration of the second substrate, comparison of normal and mutant red cell enzyme must be done under identical conditions. Purified
Robert H. Bigley, Jose O. Campos, Robert D. Koler. Kinetic Studies of Purified Leukocyte and Red Cell Pyruvate Kinase.. Ann Intern Med. 1965;63:912. doi: 10.7326/0003-4819-63-5-912_2
Download citation file:
Published: Ann Intern Med. 1965;63(5):912.
Results provided by:
Copyright © 2017 American College of Physicians. All Rights Reserved.
Print ISSN: 0003-4819 | Online ISSN: 1539-3704
Conditions of Use