Robert H. Bigley, M.D.; Jose O. Campos, M.D.; Robert D. Koler, M.D., F.A.C.P.
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Studies of purified leukocyte and red cell pyruvate kinase (PK) show different kinetics, suggesting that they are different enzymes with different mechanisms of action. In the forward reaction, the two substrates, adenosine diphosphate (ADP) and phosphoenolpyruate (PEP) combine with leukocyte PK in a noncompulsory or independent fashion with Km's very similar to those reported for rabbit muscle PK. Red cell enzyme kinetics, on the contrary, suggest compulsory combination with substrates. Since the affinity for one substrate is modified by the concentration of the second substrate, comparison of normal and mutant red cell enzyme must be done under identical conditions. Purified
Bigley RH, Campos JO, Koler RD. Kinetic Studies of Purified Leukocyte and Red Cell Pyruvate Kinase.. Ann Intern Med. ;63:912. doi: 10.7326/0003-4819-63-5-912_2
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Published: Ann Intern Med. 1965;63(5):912.
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