Nicholas A. Kefalides, M.D., Ph.D.
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The amino acid composition of the isolated, normal canine glomerular basement membrane resembled that of tendon collagen but differed in being lower in glycine, proline, and hydroxyproline but higher in hydroxylysine. The hydroxylysine: hydroxyproline ratio in basement membrane was 0.42 compared with 0.07 in collagen. Carbohydrate makes up approximately 10% of basement membrane by weight, while collagen contains 0.6% carbohydrate. Basement membrane was solubilized by reduction of disulfide bonds in 8 M urea followed by alkylation. The amino acid and carbohydrate composition of the soluble fraction was similar to that of intact basement membrane. The presence of two protein components,
Kefalides NA. The Chemical and Immunologic Properties of a New Collagen and a Glycoprotein in Glomerular Basement Membrane.. Ann Intern Med. 1966;64:1166. doi: 10.7326/0003-4819-64-5-1166_1
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Published: Ann Intern Med. 1966;64(5):1166.
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Print ISSN: 0003-4819 | Online ISSN: 1539-3704
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