CHU-TSAI CHENG, M.D.; ROBERT H. PERSELLIN, M.D., F.A.C.P.
The specificity of slide latex tests for detection of rheumatoid factor (RF) can be increased by heat-inactivating the serum (56 C, 30 minutes). Latex agglutination was decreased after heating in 187 of 372 serums studied. With few exceptions, the serums containing the heat-labile agglutinator were from patients with diseases other than rheumatoid arthritis. Forty-eight percent of serums from patients with rheumatic diseases other than rheumatoid arthritis had 1+ or greater positive latex agglutination in the unheated state, but only 9% of these were positive after heating. Serum from patients with osteoarthritis, septic arthritis, systemic lupus erythematosus, acute rheumatic fever, dermatomyositis, and Reiter's syndrome, positive before heating, became negative after incubation, increasing the specificity of the slide latex technique. The thermolabile agglutinator in serum, found to affect each of the five different slide test reagents studied, reacted in the same manner as the Clq component of complement.
CHENG C, PERSELLIN RH. Interference by Clq in Slide Latex Tests for Rheumatoid Factor. Ann Intern Med. ;75:683–686. doi: 10.7326/0003-4819-75-5-683
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Published: Ann Intern Med. 1971;75(5):683-686.
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