John R. Horn, M.D.; C. William Castor, M.D., F.A.C.P.
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A connective-tissue-activating peptide (CTAP) extracted from human cells stimulated the metabolism of synovial fibroblasts in a manner resembling rheumatoid synovitis. Metabolic changes included increased hyaluronate and lactic acid production and glucose consumption. Since CTAP had not previously been described in body fluids, plasma and joint fluid collected from patients with various rheumatic diseases was examined. Plasma or joint fluid was chromatographed on Sephadex G-50 (fine) columns. Connective-tissue-activating peptide purified from human spleen could be isolated from these columns at the same elution position used for the plasma and joint fluid samples. The joint fluid was incubated with hyaluronidase before chromatography.
Horn JR, Castor CW. Connective Tissue Activation: The Presence of Connective-Tissue-Activating Peptide in Plasma and Synovial Fluid.. Ann Intern Med. 1972;76:881. doi: 10.7326/0003-4819-76-5-881_3
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Published: Ann Intern Med. 1972;76(5):881.
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Print ISSN: 0003-4819 | Online ISSN: 1539-3704
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