Michael G. Rosenfeld, M.D.; John Mendelsohn, M.D.; Itamar B. Abrass, M.D.
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The early intracellular events initiated by antigens or phytohemagglutinin (PHA) that eventuate in transformation and mitosis of human lymphocytes are unknown. The identification of a region rich in polyriboadenylic acid (poly A) in viral and eukaryotic mRNAs facilitated the isolation of lymphocyte mRNA. Uridine labeled with 3H or [3H]adenosine was added to pure human lymphocyte cultures and poly A-rich RNA isolated after 60 minutes of incubation. Addition of PHA and dibutyryl cAMP (cyclic adenosine 3',5'-monophosphate, 1O-8M) to human lymphocyte cultures resulted in an early increase in radiolabeled poly A-rich RNA, with clear stimulation (20% to 40%) seen as early as
Rosenfeld MG, Mendelsohn J, Abrass IB. Control of Transcription in the Human Lymphocyte.. Ann Intern Med. ;78:824–825. doi: 10.7326/0003-4819-78-5-824_4
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Published: Ann Intern Med. 1973;78(5):824-825.
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Print ISSN: 0003-4819 | Online ISSN: 1539-3704
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