MICHAEL E. ROSS, M.D.; ROBERT O. WOLF, D.D.S.
To the editor: The problems in the clinical interpretation of hyperamylasemia are well summarized in a recent editorial (1); however, this editorial does not give an adequate picture of the situations in which the serum amylase may be clinically valuable.
Polyacrylamide gel electrophoresis can reliably separate serum or urine samples into their constituent isoamylases, and when this is done the pancreatic isoamylases are separated from the isoamylases of salivary gland origin (2). The isoamylases can then be analyzed either qualitatively, by examination of zymograms (3), or quantitatively, by fractionating the gel and assaying the amylase content of each slice (4).
ROSS ME, WOLF RO. Pancreatic Isoamylases. Ann Intern Med. ;81:566. doi: 10.7326/0003-4819-81-4-566
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Published: Ann Intern Med. 1974;81(4):566.
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