K. H. WONG, Ph.D.; C. W. MOSS, Ph.D.; D. H. HOCHSTEIN, D.P.H.; R. J. ARKO, D.V.M.; W. O. SCHALLA, M.S.
The Legionnaires' disease (LD) bacterium is a gram-negative organism whose "endotoxicity" appears to differ in several respects from the classic endotoxicity generally associated with gram-negative bacteria. Discrepancies were noted between the high activity of LD bacteria in gelating limulus lysate in vitro and their low pyrogenicity in rabbits. Further in-vivo biologic tests indicated that LD bacteria were relatively weak in "endotoxicity." Analysis of LD bacterial cells and their cellular fractions by gas-liquid chromatography indicated that LD bacteria did not contain hydroxy fatty acids commonly associated with lipid A of endotoxin. The branched-chain fatty acids that were characteristic of LD bacteria were associated with the cell envelope, and were readily extracted into organic solvents without prior saponification. The presence of 2-keto-3-deoxyoctonate in LD bacteria and cell extracts was detected by a microassay method but remains to be confirmed with gas-liquid chromatography and mass spectrometry. The active principle of "endotoxicity" in LD bacteria may be a new type of bacterial lipopolysaccharide.
K. H. WONG, C. W. MOSS, D. H. HOCHSTEIN, R. J. ARKO, W. O. SCHALLA. "Endotoxicity" of the Legionnaires' Disease Bacterium. Ann Intern Med. 1979;90:624–627. doi: 10.7326/0003-4819-90-4-624
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Published: Ann Intern Med. 1979;90(4):624-627.
Infectious Disease, Pulmonary/Critical Care.
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