WERNER A. JANSSEN, S.M.; KENNETH W. HEDLUND, M.D.
To the editor: The causative organisms in Legionnaires' disease, Pontiac fever, and Pittsburgh pneumonia are rod-shaped bacteria that are refractory to most bacteriologic and histologic stains. They are considered to be gram-negative, although they usually stain very weakly even after prolonged exposure to safranin in the Gram staining procedure. The initial successful demonstration of Legionella pneumophila in culture smears was made by McDade and colleagues (1) using the Giménez stain and in human lung tissue sections by Chandler and associates (2) using a modified Dieterle silver impregnation procedure. These staining procedures are still the most widely used in studies of
JANSSEN WA, HEDLUND KW. Giemsa Stain for Legionella pneumophila and TATLOCK Bacterium. Ann Intern Med. ;94:413–414. doi: 10.7326/0003-4819-94-3-413_2
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Published: Ann Intern Med. 1981;94(3):413-414.
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