JOHN A. RANKIN, M.D.; K. RANDALL YOUNG JR., M.D.
To the editor: Recently, Macher and coworkers (1) have described the usefulness of gram-staining touch preparations of lung biopsy material for the diagnosis of Pneumocystis carinii pneumonia in patients with the acquired immunodeficiency syndrome. This method is more rapid than the conventional staining techniques used to reveal the cystic form of this organism, and the sporozoites are easily visible within "coagula" seen in the touch preparations. Their report prompted us to use the Gram stain on freshly obtained (< 1 hour old) cellular phase material obtained by bronchoalveolar lavage from three patients with suspected P. carinii pneumonia (subsequently confirmed by
RANKIN JA, YOUNG KR. Gram Staining of Pneumocystis Sporozoites. Ann Intern Med. ;100:919. doi: 10.7326/0003-4819-100-6-919_1
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Published: Ann Intern Med. 1984;100(6):919.
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