DONALD S. BURKE, M.D.; BRENDA L. BRANDT, M.S.; ROBERT R. REDFIELD, M.D.; TUN-HOU LEE, Ph.D.; RICHARD M. THORN, Ph.D.; GERALD A. BELTZ, Ph.D.; CHUNG-HO HUNG, Ph.D.
To detect human immunodeficiency virus (HIV) antibodies in a simple enzyme-linked immunoassay (CBre3-EIA), we used an Escherichia coli-expressed polypeptide antigen, representing the carboxy-terminal third of the external membrane glycoprotein gene fused with the amino-terminal half of the transmembrane glycoprotein gene. Over a 3-month period, 2707 consecutive serum samples referred for confirmatory testing for human T-lymphotrophic virus type III (HTLV-III) antibodies were evaluated by both Western blot and CBre3-EIA. On a single determination for each sample, the CBre3-EIA was found to have an estimated sensitivity (99.9%) and specificity (99.1%) similar or superior to the more cumbersome Western blot method. This study shows that all HIV-seropositive subjects have antibodies to the virus envelope protein; no other virus antigens are required for construction of highly sensitive immunoassays.
BURKE DS, BRANDT BL, REDFIELD RR, LEE T, THORN RM, BELTZ GA, et al. Diagnosis of Human Immunodeficiency Virus Infection by Immunoassay Using a Molecularly Cloned and Expressed Virus Envelope Polypeptide: Comparison to Western Blot on 2707 Consecutive Serum Samples. Ann Intern Med. ;106:671–676. doi: 10.7326/0003-4819-106-5-671
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Published: Ann Intern Med. 1987;106(5):671-676.
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