ALEC E. WITTEK, M.D., C.M.; MICHAEL A. PHELAN, Ph.D.; MARTHA A. WELLS, B.S.; LUBA K. VUJCIC, M.S.; JAY S. EPSTEIN, M.D.; H. CLIFFORD LANE, M.D.; GERALD V. QUINNAN Jr., M.D.
A sensitive enzyme immunoassay was developed for detecting human immunodeficiency virus (HIV) core antigen. Assay sensitivity was 3.67 pmol/L of purified HIV core protein, and 1 or 100 in-vitro infectious units/mL of HIV in purified virus preparations or cell culture supernatants, respectively. Enzyme immunoassay sensitivity exceeded that of reverse transcriptase assay by 1000-fold. Core antigen was detected in whole plasma from 41% of symptomatic subjects and 13% of asymptomatic subjects seropositive for HIV. After plasma fractionation, antigenemia was found in 60% of symptomatic subjects and in 33% of asymptomatic subjects seropositive for HIV. Fifty-seven percent of samples from which HIV could be isolated in lymphocyte culture had detectable quantities of core antigen in plasma. However, at least 87% of samples with measurable antigen in plasma had HIV isolated from lymphocyte cultures. Antigenemia was associated with reduced T-cell number and symptomatic disease, and may be a useful marker for disease progression.
WITTEK AE, PHELAN MA, WELLS MA, VUJCIC LK, EPSTEIN JS, LANE HC, et al. Detection of Human Immunodeficiency Virus Core Protein in Plasma by Enzyme Immunoassay: Association of Antigenemia with Symptomatic Disease and T-Helper Cell Depletion. Ann Intern Med. ;107:286–292. doi: 10.7326/0003-4819-107-2-286
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Published: Ann Intern Med. 1987;107(3):286-292.
HIV, Infectious Disease.
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