Fabrizio Spinozzi, MD; Elisabetta Agea, MD; Onelia Bistoni, BSc; Nicolino Forenza, MD; Alessandro Monaco, MD; Gabrio Bassotti, MD, PhD; Ildo Nicoletti, MD; Carlo Riccardi, MD; Fausto Grignani, MD; Alberto Bertotto, MD
To test the hypothesis that allergen-specific, steroid-sensitive γδ T lymphocytes are increased in bronchoalveolar lavage fluid of patients with asthma.
The outpatient allergy services at the University of Perugia, Perugia, Italy.
12 untreated atopic patients (6 children and 6 adults) with mildly symptomatic chronic asthma were studied. Bronchoalveolar lavage fluid from 10 healthy nonsmoking volunteers and age-matched children with cystic fibrosis (n = 5) or anatomic malformation of the airways (n = 4) served as control samples.
Three patients received treatment with deflazacort, 60 mg twice daily, for 1 week.
CD3+, CD4+, and CD8+ T cells from patients and controls were examined by two-color flow cytometry for coexpression of Vδ1 and Vδ2 isoforms of the γδ T-cell receptor. In vitro pulmonary γδ T-cell proliferation in response to a specific allergen, the apoptotic death of these cells after incubation with 10−7 M dexamethasone, and bronchoalveolar lavage fluid T-lymphocyte composition before and after 1 week of deflazacort therapy were evaluated in 3 Dermatophagoides pteronyssinus-sensitive patients.
The proportion of γδ T lymphocytes, primarily CD4+ or CD4− CD8− cells, was higher in asthmatic patients than in controls (P < 0.05 by one-way analysis of variance). Most lung γδ CD4+ lymphocytes expressed the γδ T-cell receptor Vδ1 chain. These cells proliferated in response to allergen stimulation, underwent steroid-induced apoptosis in vitro, and disappeared after systemic steroid treatment.
Allergen-specific, steroid-sensitive γδ T cells may be one of the cellular components involved in the airway inflammation that characterizes allergic bronchial asthma.
Fabrizio Spinozzi, Elisabetta Agea, Onelia Bistoni, Nicolino Forenza, Alessandro Monaco, Gabrio Bassotti, et al. Increased Allergen-Specific, Steroid-Sensitive γδ T Cells in Bronchoalveolar Lavage Fluid from Patients with Asthma. Ann Intern Med. 1996;124:223–228. doi: 10.7326/0003-4819-124-2-199601150-00005
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Published: Ann Intern Med. 1996;124(2):223-228.
Asthma, Pulmonary/Critical Care.
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