Roger M. Morrell, M.D.
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The intracellular site and mode of action of hypothalamic releasing factors for anterior pituitary hormones are unknown. Labeled (125I)-rabbit-antihuman growth hormone (HGH) was incubated with bovine anterior pituitary supernatant and shown on sucrose density gradient analysis to have bound to a specific polyribosomal (polysomal) peak. The bound radioactivity was liberated into the ribosomal monomer peak by pancreatic ribonuclease that also abolished the polysomes. Treatment with ovine hypothalamic extract containing growth-hormone releasing factor (SRF) resulted in a shift of radioactivity into a different (soluble) portion of the gradient. The latter treatment left the polysomes intact, indicating minimal contamination with pancreatic ribonuclease.
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Morrell RM. Release of Bound Antihuman Growth Hormone from Bovine Anterior Pituitary Polyribosomes by Ribonuclease and Hypothalamic Extract.. Ann Intern Med. 1969;70:1063–1064. doi: 10.7326/0003-4819-70-5-1063_3
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Published: Ann Intern Med. 1969;70(5):1063-1064.
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Print ISSN: 0003-4819 | Online ISSN: 1539-3704
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