CLAIRE V. BROOME, M.D.; WILLIAM B. CHERRY, Ph.D.; WASHINGTON C. WINN, M.D.; BRUCE R. MacPHERSON, M.D.
A blind reading of direct immunofluorescent staining of sputum smears was positive in five of 21 patients with Legionnaires' disease and none of 47 control patients. Positive slides showed from eight organisms per smear (one slide) to 20 to 100 organisms per oil-immersion (magnification, 100) field (three slides). Two of the positive specimens were obtained through an endotracheal tube, one at bronchoscopy, and the other positive slides were from expectorated specimens. In addition, two open-lung biopsies and a bronchial lavage sediment from three confirmed cases of Legionnaires' disease were positive by direct immunofluorescent staining for Legionnaires' disease bacterium.
Learn more about subscription options.
Register Now for a free account.
BROOME CV, CHERRY WB, WINN WC, MacPHERSON BR. Rapid Diagnosis of Legionnaires' Disease by Direct Immunofluorescent Staining. Ann Intern Med. 1979;90:1-4. doi: 10.7326/0003-4819-90-1-1
Download citation file:
Published: Ann Intern Med. 1979;90(1):1-4.
Infectious Disease, Pulmonary/Critical Care.
Results provided by:
Copyright © 2017 American College of Physicians. All Rights Reserved.
Print ISSN: 0003-4819 | Online ISSN: 1539-3704
Conditions of Use
This PDF is available to Subscribers Only