CHESTER C. WOOD, M.D.; ALAN E. WILLIAMS, Ph.D.; JAMES G. McNAMARA, M.D.; JOSEPHINE A. ANNUNZIATA, B.S.; PAUL M. FEORINO, Ph.D.; CHRISTOPHER O. CONWAY, M.S.
In a 6-month study, antibody levels against the human immunodeficiency virus (HIV) were determined in intravenous gammaglobulin preparations and 45 serum samples from 20 patients on gammaglobulin therapy. All 10 lots of a reduced and alkylated preparation and 4 of 8 lots of a pH4/pepsin-treated preparation were seropositive by enzyme-linked immunosorbent assay (ELISA). By Western blot analysis, 8 of 10 lots of the reduced and alkylated preparation and 3 of 8 lots of the pH4/pepsin-treated preparation were positive. Before gammaglobulin infusion, 2 of 45 preinfusion samples were seropositive by ELISA but seronegative by Western blot. After infusion, 15 of 45 samples were seropositive by ELISA, and 8 had antibody against p24 by Western blot. Seropositivity persisted for less than 1 month. Cultures of HIV-positive intravenous gammaglobulin lots were negative for reverse transcriptase activity or viral antigen expression. These results suggest that current methods of preparation either exclude or inactivate HIV.
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WOOD CC, WILLIAMS AE, McNAMARA JG, ANNUNZIATA JA, FEORINO PM, CONWAY CO. Antibody Against the Human Immunodeficiency Virus in Commercial Intravenous Gammaglobulin Preparations. Ann Intern Med. 1986;105:536–538. doi: 10.7326/0003-4819-105-4-536
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Published: Ann Intern Med. 1986;105(4):536-538.
HIV, Infectious Disease.
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Print ISSN: 0003-4819 | Online ISSN: 1539-3704
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