Richard C. Hubbard, MD; Mark L. Brantly, MD; Stephanie E. Sellers, BS; Marc E. Mitchell, MD; Ronald G. Crystal, MD
Study Objective: To determine if aerosolization of purified human plasma α1-antitrypsin is an effective means for increasing lower respiratory anti-neutrophil-elastase defenses in α1-antitrypsin deficiency.
Design: Nonrandomized, before-and-after trial with a 7-day treatment period. Companion studies in animals to determine lung epithelial permeability to α1-antitrypsin.
Patients: Twelve patients with homozygous Z-type α1-antitrypsin deficiency and mild to moderate emphysema.
Interventions: Aerosol administration of human plasma α1-antitrypsin, 100 mg every 12 hours for 7 days. Single, 100-mg aerosol dose to anesthetized sheep with indwelling thoracic lymph duct catheters for direct assessment of lung permeability.
Measurements and Main Results: Treatment resulted in increased α1-antitrypsin levels in the lung epithelial lining fluid (0.28 ±0.07 µM before therapy to 5.86 ±1.03 µM after therapy) and increased anti-neutrophil-elastase capacity (0.78 ±0.38 ΩM before therapy to 4.16 ±0.95 µM after therapy). Aerosolized α1-antitrypsin diffused across the respiratory epithelium and entered lung interstitial lymph (in sheep) and reached the systemic circulation (in sheep and humans). No side effects were noted.
Conclusion: Short-term aerosol administration of human plasma α1-antitrypsin to patients with α1-antitrypsin deficiency is safe and feasible, resulting in a return to normal of anti-neutrophil-elastase defenses in the lower respiratory tract. The aerosol approach, therefore, merits serious longterm evaluation as an alternative to other parenteral forms of administering therapeutic proteins.
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Hubbard RC, Brantly ML, Sellers SE, Mitchell ME, Crystal RG. Anti-Neutrophil-Elastase Defenses of the Lower Respiratory Tract in α1-Antitrypsin Deficiency Directly Augmented with an Aerosol of α1-Antitrypsin. Ann Intern Med. 1989;111:206–212. doi: 10.7326/0003-4819-111-3-206
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Published: Ann Intern Med. 1989;111(3):206-212.
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Print ISSN: 0003-4819 | Online ISSN: 1539-3704
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