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Studies on Inhibition of Insulin Activity.

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By KARELITZ SAMUEL, COHEN PHILIP, LEADER SYDNEY D..  (Proc. Soc. Exper. Biol. and Med.. , October, 1928; , p. 11.).

Ann Intern Med. 1928;2(6):578. doi:10.7326/0003-4819-2-6-578_1
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This investigation on the inhibitory effect of blood on insulin activity was incited by the study in 1925 of a diabetic child with refractory periods to insulin. The work was done with rabbits. One physiologic or 3 clinical units of insulin were mixed with the substance investigated and incubated at 37° C. from 1 to 2 hours, then injected subcutaneously into rabbits weighing 2 kilos, starved during the preceding 24 hours. Hourly blood sugar determination and clinical observations were made. The results summarized were as follows: 2-4 cc. of human plasma caused hardly any inhibition of insulin action; 5 cc.




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