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Application of Serum Microimmunoelectrophoresis to the Clinical Evaluation of Patients with Hypersensitivity Vasculitis and Other Allergic Diseases.

Charles E. Buckley III, M.D.; and Herbert O. Sieker, M.D., F.A.C.P.
Ann Intern Med. 1964;60(4):730-731. doi:10.7326/0003-4819-60-4-730_3
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Microimmunoelectrophoresis of mixtures of proteins has been demonstrated to be a useful tool for the identification of antigenically distinguishable normal and abnormal plasma proteins in the dysgammaglobulinemias and in malignancies of the reticuloendothelial system. Despite its potential usefulness, it has not been applied as an aid in the clinical evaluation of diseases in which allergic diathesis occurs. The normal and abnormal plasma proteins in 75 patients with illness involving alterations in immunity or the immune proteins were examined by microimmunoelectrophoresis. A modification of the method of Scheidigger using 2 per cent Noble agar and Tris-chloride, pH 8.6, 0.05 ionic strength


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