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Routine Immunohistology as an Aid in the Diagnosis and Prognosis of Renal Diseases.

Kurt Lange, M.D., F.A.C.P.; Gerhard Treser, M.D.; Edward Wasserman, M.D.; and Inge Sagel, M.D.
Ann Intern Med. 1964;60(4):738. doi:10.7326/0003-4819-60-4-738_2
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Immunohistologic staining with fluorescein labelled antibodies against gamma globulin, type 12 streptococcal antigens, serum complement, as well as its components C′1 and C′3 (β1C and β1A) were carried out as a routine procedure on all kidney biopsies of patients with alleged or proven renal diseases.

Experimentally, it could be shown that neither gamma globulin nor complement or its components accumulate in renal lesions on the basis of penetration into inflamed areas alone, but that such staining was always caused by complement binding antigen-antibody reactions.

Patients with acute glomerulonephritis invariably showed an intense staining for gamma globulin and complement and its


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