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Plasma Luteinizing Hormone Levels in Normal Adults and in Patients with Pituitary and Gonadal Dysfunction: Determination by Radioimmunoassay.

Don S. Schalch, M.D.; Louyse Lee, B.S.; Robert C. Boon, M.D.; Seymour Reichlin, M.D.; and Albert F. Parlow, Ph.D.
Ann Intern Med. 1967;66(5):1028-1029. doi:10.7326/0003-4819-66-5-1028_3
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A sensitizing radioimmunoassay for luteinizing hormone (LH) has been developed using a highly purified preparation of human pituitary LH. By this technique, the plasma LH concentration measured daily during normal menstrual cycles in eight healthy women averaged 1.2 ± 0.1 mµg/ml (mean ± SE) in the pre- and postovulatory phases but rose abruptly at the time of ovulation to a level of 9.9 ± 0.9 mµg/ml. This midcycle peak was completely obliterated by oral ovulatory suppressants but reappeared in the first month after the discontinuation of medication. Plasma LH levels in ten postmenopausal women were markedly elevated, ranging between 2.7


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