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Clinical Implications of Total and Isoenzyme Lactic Dehydrogenase Activity in Sputum.

Felix Wroblewski, M.D., F.A.C.P.; Ida Levine, M.D.; Augusta Flieg, B.S.; and M. Sansur, Ph.D.
Ann Intern Med. 1968;68(5):1187. doi:10.7326/0003-4819-68-5-1187_1
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Lactio dehydrogenase (LDH) activity was found to be present in all 64 sputum specimens examined. Total LDH activity appears to be related to the clinical severity of the bronchopulmonary disturbance but not to the etiologic factors. The slowest migrating LDH isoenzymes (LDH1 and LDH2) were present in all the specimens examined. LDH3, LDH4, and LDH5 activity appeared in increasing amounts in the presence of progressive, or clinically severe, or both, bronchopulmonary disease. Since human bronchopulmonary tissue has a preponderance of the three fastest electrophoretically migrating LDH isoenzymes, the presence of LDH3, LDH4, and LDH5 in sputum in the course of


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