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Amylase in Diagnosis of Pancreatic Disease

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Department of Medicine, University of California Irvine Medical Center; Orange, California

Ann Intern Med. 1978;88(6):838-839. doi:10.7326/0003-4819-88-6-838
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A recent major advance in the diagnostic use of amylase in pancreatic disease is the so-called isoamylase analysis (1). Using various methods (chiefly electrophoresis, isoelectric focusing, and column chromatography), one can identify two major and a variable number of minor amylase peaks in human serum and urine (1, 2). The major peak in serum and urine corresponds in mobility or isoelectric position with the principal amylase peak found in purified human pancreatic extract using similar methods of study; this peak has been designated "P-type." The major peak that corresponds in position to the principal peak found in parotid gland or


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