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Otto Folin, America's First Clinical Biochemist

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By Meites Samuel. . 428 pages. American Association for Clinical Chemistry, Inc., Washington, D.C: , 1989.. $55.00.


Ann Intern Med. 1989;111(3):265. doi:10.7326/0003-4819-111-3-265_2
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Anyone familiar with the measurement of protein by the Lowry method-the most frequently used analytical method in history to date-knows that the blue color is produced in an alkaline copper solution by the addition of the Folin phenol reagent. Otto Folin, born in Sweden, was one of the great pioneers of clinical biochemistry and spent his entire working life devising and improving analytical methods-mainly for nitrogenous compounds-which could be applied to the study of the composition of urine and blood. He introduced colorimetric methods for this purpose, and applied the Jaffe reaction to the measurement of urinary creatinine, showing that

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