0

The full content of Annals is available to subscribers

Subscribe/Learn More  >
Articles |

Tumor Cell Lines Established in Vitro: An Independent Prognostic Factor for Survival in Non-Small-Cell Lung Cancer

Henry Stevenson, MD; Adi F. Gazdar, MD; Ruby Phelps, AAS; R. Ilona Linnoila, MD; Daniel C. Ihde, MD; Bimal Ghosh, MD; Thomas Walsh, MD; Edward L. Woods, MD; Herbert Oie, PhD; Teresa O'Connor, MPH; Robert Makuch, PhD; Barnett S. Kramer, MD; and James L. Mulshine, MD
[+] Article and Author Information

Grant Support: By the G. Harold and Leila Y. Mathers Charitable Trust.

Requests for Reprints: James L. Mulshine, MD, Biotherapy Section, NCI-Navy Medical Oncology Branch, National Naval Medical Center, Building 8, Room 5101, Bethesda, MD 20814.

Current Author Addresses: Drs. Stevenson, Gazdar, Linnoila, Ihde, Ghosh, Walsh, Oie, Kramer, Mulshine, and Ms. Phelps: The NCI-Navy Medical Oncology Branch, National Cancer Institute, National Naval Medical Center, and Uniformed Services University of the Health Sciences, Bethesda, MD 20814.

Dr. Makuch and Ms. O'Connor: Department of Epidemiology and Public Health, Yale University, New Haven, CT 06510.


Ann Intern Med. 1990;113(10):764-770. doi:10.7326/0003-4819-113-10-764
Text Size: A A A

Objective: To determine the relation between in-vitro establishment of tumor cell lines and survival in patients with non-small-cell lung cancer.

Design: Cohort study.

Setting: Single-institution tertiary care center.

Patients: One hundred twenty-three consecutive patients with non-small-cell lung cancer from whom a viable tumor specimen could be obtained.

Intervention: Tumor tissue was removed at the time of entry into a therapeutic protocol. The tumor tissue was processed in the laboratory for attempted cell-line establishment. Patients classified as potentially curable (stages I, II, and IIIA) were treated with surgical resection, radiation therapy, or a combination. Patients suitable for palliative therapy only (stages HIB and IV) were treated with radiation therapy with or without chemotherapy. Chemotherapy was based on in-vitro drug sensitivity when available. Cell-line establishment was correlated to clinical outcome.

Measurements and Main Results: Univariate analysis of survival was done using the log-rank test; multivariate analysis was done by Cox modeling step-up and step-down techniques. Cell lines were established from the tumor specimens of 25 patients (20%). Those patients experienced a median survival of 7 months compared with 18 months in patients from whom cell lines could not be established (P < 0.001). In the 61 patients with potentially curable disease, 8 patients (13%) with cell lines established had a median survival of 8 months compared with 32 months for those without cell lines established (P = 0.001). In the 62 palliative group patients, the median survival of the 17 patients (27%) from whom tumor cell lines were established was 5 months compared with 7 months for those without cell lines (P = 0.15). Multivariate analysis in both groups showed cell-line establishment to be a significant indicator of prognosis (P < 0.0001 for curable group; P < 0.01 for palliative group).

Conclusion: In-vitro tumor growth is related to decreased patient survival, which in turn reflects the biologic aggressiveness of cancers giving rise to these tumor cell lines.

Figures

Tables

References

Letters

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Comments

Submit a Comment
Submit a Comment

Summary for Patients

Clinical Slide Sets

Terms of Use

The In the Clinic® slide sets are owned and copyrighted by the American College of Physicians (ACP). All text, graphics, trademarks, and other intellectual property incorporated into the slide sets remain the sole and exclusive property of the ACP. The slide sets may be used only by the person who downloads or purchases them and only for the purpose of presenting them during not-for-profit educational activities. Users may incorporate the entire slide set or selected individual slides into their own teaching presentations but may not alter the content of the slides in any way or remove the ACP copyright notice. Users may make print copies for use as hand-outs for the audience the user is personally addressing but may not otherwise reproduce or distribute the slides by any means or media, including but not limited to sending them as e-mail attachments, posting them on Internet or Intranet sites, publishing them in meeting proceedings, or making them available for sale or distribution in any unauthorized form, without the express written permission of the ACP. Unauthorized use of the In the Clinic slide sets will constitute copyright infringement.

Toolkit

Buy Now

to gain full access to the content and tools.

Want to Subscribe?

Learn more about subscription options

Advertisement
Related Articles
Journal Club
Related Point of Care
Topic Collections
PubMed Articles
Breast Cancer Stem Cells, EMT and Therapeutic Targets. Biochem Biophys Res Commun Published online Sep 24, 2014.;
Forgot your password?
Enter your username and email address. We'll send you a reminder to the email address on record.
(Required)
(Required)