0

The full content of Annals is available to subscribers

Subscribe/Learn More  >
Articles |

Transmission of Hepatitis A to Patients with Hemophilia by Factor VIII Concentrates Treated with Organic Solvent and Detergent To Inactivate Viruses

Pier Mannuccio Mannucci, MD; Susan Gdovin, PhD; Alessandro Gringeri, MD; Massimo Colombo, MD; Alfonso Mele, MD; Nicola Schinaia, MD; Nicola Ciavarella, MD; Suzanne U. Emerson, PhD; Robert H. Purcell, MD, Italian Collaborative Group*
[+] Article and Author Information

From Angelo Bianchi Bonomi Hemophilia and Thrombosis Center and Institute of Internal Medicine, IRCCS Maggiore Hospital and University of Milan, Milan, Italy; National Institutes of Health, Bethesda, Maryland; University of Maryland, College Park, Maryland; Istituto Superiore di Sanita, Rome, Italy; Hemophilia and Thrombosis Center, Bari, Italy. The Italian Collaborative Group includes A. Mele, N. Schinaia, G. Gentili, C. Collotti, A. Ghirardini, and R. Bellocco (Istituto Superiore di Sanita, Rome, Italy); the following staff from the Hemophilia Centers: P. M. Mannucci, A. Gringeri, M. Colombo, E. Santagostino, A. Zanetti (Milano); F. Rodeghiero, E. Di Bona (Vicenza); G. Tagariello (Castelfranco Veneto); G. Gamba (Pavia); V. De Rosa (Bologna); M. Morfini (Florence); M. Berrettini (Perugia); G. Mariani, G. Mazzucconi, A. Chistolini (Rome); N. Ciavarella, M. Schiavoni, F. A. Scaraggi (Bari); G. Rossetti (Trento); R. De Biasi and A. Rocino (Naples). * Drs. Mannucci and Gdovin contributed equally to the research culminating in this article and should be acknowledged as coequal first authors. Requests for Reprints: P.M. Mannucci, MD, Via Pace 9, 20122 Milano, Italy. Acknowledgments: The authors thank the Italian Hemophilia Center Director, who supplied detailed information on hemophilic care and controls. They also thank Dr. Stanley Lemon for providing nucleotide sequences of wild-type HAV strains; Dr. Sugantha Govindarajan for evaluating liver biopsy specimens; Dr. Sergei Tsarev for doing assays for antibody to hepatitis E virus; Dr. David Alling for performing statistical analysis; Doris Wong for managing clinical materials; and Laurie Moore for providing editorial assistance Grant Support: In part by a grant from the World Health Organization Programme for Vaccine and Development and contract no. NO1-AI-05069 to Bioqual, Inc., Rockville, Maryland.


Copyright ©2004 by the American College of Physicians


Ann Intern Med. 1994;120(1):1-7. doi:10.7326/0003-4819-120-1-199401010-00001
Text Size: A A A

Objective: To determine whether an outbreak of hepatitis A virus (HAV) infection that occurred in 52 patients with hemophilia in Italy was acquired through infusion of contaminated factor VIII or through environmental enteric transmission.

Design: A case–control study and a molecular analysis of HAV sequences from implicated lots of factor VIII and from infected patients.

Patients: The first 29 patients with hemophilia and jaundice in whom hepatitis A developed were compared with one to three matched controls with hemophilia but no jaundice.

Measurements: Type of concentrate and batches infused, number of doses, contacts with persons who had jaundice or hepatitis A, travel abroad to countries reported to have a high attack rate for hepatitis A, and consumption of raw shellfish. Hepatitis A viral sequences sought by polymerase chain reaction in lots of factor VIII and in serial serum samples from two patients with hemophilia in whom hepatitis A developed. Amplification by polymerase chain reaction of cDNA transcribed with reverse transcriptase from matched sets of factor VIII and recipient serum samples. Determination of nucleotide sequence of amplified hepatitis A virus genome.

Main Results: Case patients were neither more nor less likely than controls to have traveled to high-risk countries, consumed raw shellfish, or had contact with persons with jaundice. Case patients were more likely than controls to have received a factor VIII concentrate treated with a solvent-detergent mixture to inactivate viruses (odds ratio, ∞; 95% CI, 4.5 to ∞) and to have had larger infusions of the concentrate during the presumed HAV incubation period (odds ratio, 8.54; CI, 2.78 to 27.5). Hepatitis A viral sequences were found in 5 of 12 tested lots of factor VIII. Genomic sequences of HAV obtained for two matched sets of factor VIII and recipient serum samples were identical within each set but different for the two sets.

Conclusion: Hepatitis A was transmitted by a factor VIII concentrate treated by a virucidal method (solvent-detergent) that ineffectively inactivates nonenveloped viruses.

Figures

Grahic Jump Location
Figure 1.
Comparison of hepatitis A virus nucleotide sequences from VP3 region (A) or VP1/2A region (B) amplified from factor VIII concentrate and the patient's serum sample.

The location of nucleotide in HAV genome is shown at the top. All strains were compared with nucleotide sequences identified from factor VIII lots 0559910102 and 0556900101. HM-175 = human laboratory strain. AGM-27 = simian laboratory strain. Dashes represent identity. Differences are shown by the appropriate base letter.

Grahic Jump Location

Tables

References

Letters

NOTE:
Citing articles are presented as examples only. In non-demo SCM6 implementation, integration with CrossRef’s "Cited By" API will populate this tab (http://www.crossref.org/citedby.html).

Comments

Submit a Comment
Submit a Comment

Summary for Patients

Clinical Slide Sets

Terms of Use

The In the Clinic® slide sets are owned and copyrighted by the American College of Physicians (ACP). All text, graphics, trademarks, and other intellectual property incorporated into the slide sets remain the sole and exclusive property of the ACP. The slide sets may be used only by the person who downloads or purchases them and only for the purpose of presenting them during not-for-profit educational activities. Users may incorporate the entire slide set or selected individual slides into their own teaching presentations but may not alter the content of the slides in any way or remove the ACP copyright notice. Users may make print copies for use as hand-outs for the audience the user is personally addressing but may not otherwise reproduce or distribute the slides by any means or media, including but not limited to sending them as e-mail attachments, posting them on Internet or Intranet sites, publishing them in meeting proceedings, or making them available for sale or distribution in any unauthorized form, without the express written permission of the ACP. Unauthorized use of the In the Clinic slide sets will constitute copyright infringement.

Toolkit

Buy Now

to gain full access to the content and tools.

Want to Subscribe?

Learn more about subscription options

Advertisement
Related Articles
Related Point of Care
Topic Collections
PubMed Articles
Forgot your password?
Enter your username and email address. We'll send you a reminder to the email address on record.
(Required)
(Required)