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Screening for HIV

Ned Calonge, MD, MPH; and Diana B. Petitti, MD, MPH
[+] Article and Author Information

From U.S. Preventive Services Task Force, Denver, CO 80246, and Pasadena, CA 91888.


Potential Financial Conflicts of Interest: None disclosed.


Ann Intern Med. 2005;143(12):916-917. doi:10.7326/0003-4819-143-12-200512200-00013
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Inexpensive CD4 counting for the developing world
Posted on December 25, 2005
Prasanta Padhan
JIPMER,Pondicherry,India.605006
Conflict of Interest: None Declared

Dear Editor,

With increasing number and antiretroviral therapy of HIV-positive patients in developing countries, their monitoring is essential by regular CD-4 cell counts in peripheral blood which is quite expensive if done by flow cytometry method.Recently, William Rodriguez and his colleagues recently developed a microchip-based detection system, called an electronic taste chip, that can detect chemicals and proteins in solution. Each chip contains microspheres in a small chamber through which fluid passes "” whole blood, for example. The microspheres are coated with monoclonal antibodies that attach to the surface proteins of lymphocytes such as CD4 as they pass through the chamber.The chip array rests atop a fluorescent microscope connected to a charge-coupled device (CCD). The CD4 cells tagged with microspheres can be distinguished via this CCD camera and counted by computer software(1).HIV-positive patients in the developing world requires a cheaper alternative for counting CD4 cells, and thus microchip based technique could be a promising choice in future. Reference: (1)Rodriguez WR, Christodoulides N, Floriano PN, Graham S, Mohanty S, Dixon M, et al. A microchip CD4 counting method for HIV monitoring in resource- poor settings. PLoS Med 2005;2(7):e182.

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