Fusobacterium-Positive and Streptococcal-Positive Pharyngitis

James R. Uhl; Daniel R. Gustafson; Stefanea L. Rucinski; Robin Patel

doi:10.7326/L15-5099-2

Letters |16 June 2015

Fusobacterium-Positive and Streptococcal-Positive Pharyngitis

James R. Uhl, MS; Daniel R. Gustafson, BS; Stefanea L. Rucinski, MT (ASCP); Robin Patel, MD

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TO THE EDITOR:

We developed a real-time polymerase chain reaction (PCR) assay to detect F. necrophorum in throat swabs; assay evaluation yielded findings that support and expand those of Centor and colleagues (1). Between November 2010 and January 2011, a total of 239 swabs submitted for Streptococcus pyogenes PCR testing (2) was evaluated for F. necrophorum using culture and PCR targeting the phosphotransferase system I gene using fluorescence resonance energy transfer hybridization probes. For PCR, swabs were cut into 50 µL of 0.1-mm glass beads and 600 µL of Tris buffer solution and processed using an Eppendorf ThermoMixer (Eppendorf AG) for 6 minutes at 95 °C at 1400 revolutions per minute. For culture, 5 mL of fastidious anaerobe broth with 1 µg/mL of norfloxacin, 4 µg/mL of vancomycin, and 3 µg/mL of josamycin were added to swab pledgets, which were then incubated anaerobically at 37 °C for 2 days and subcultured to fastidious anaerobe agar plates with the same supplementation. Colonies consistent with F. necrophorum were identified by PCR and sequencing of a portion of the 16S ribosomal RNA gene.

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Letters |16 June 2015

Fusobacterium-Positive and Streptococcal-Positive Pharyngitis

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Letters |16 June 2015

Fusobacterium-Positive and Streptococcal-Positive Pharyngitis

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