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Letters |16 June 2015

Fusobacterium-Positive and Streptococcal-Positive Pharyngitis

James R. Uhl, MS; Daniel R. Gustafson, BS; Stefanea L. Rucinski, MT (ASCP); Robin Patel, MD

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TO THE EDITOR:
We developed a real-time polymerase chain reaction (PCR) assay to detect F. necrophorum in throat swabs; assay evaluation yielded findings that support and expand those of Centor and colleagues (1). Between November 2010 and January 2011, a total of 239 swabs submitted for Streptococcus pyogenes PCR testing (2) was evaluated for F. necrophorum using culture and PCR targeting the phosphotransferase system I gene using fluorescence resonance energy transfer hybridization probes. For PCR, swabs were cut into 50 µL of 0.1-mm glass beads and 600 µL of Tris buffer solution and processed using an...
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Uhl JR, Gustafson DR, Rucinski SL, et al. Fusobacterium-Positive and Streptococcal-Positive Pharyngitis. Ann Intern Med. 2015;162:876–877. doi: https://doi.org/10.7326/L15-5099-2

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Published: Ann Intern Med. 2015;162(12):876-877.

DOI: 10.7326/L15-5099-2

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Print ISSN: 0003-4819 | Online ISSN: 1539-3704

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