R. A. Johnson, M.D.; Thomas M. Blake, M.D., F.A.C.P.
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Study of the lymphatic system has been handicapped by limitations of available methods. Meticulous injection techniques have been the primary means of investigation. In the present study of cardiac lymphatics, a simple and effective method is used that requires neither live specimens nor special preparation. A 1% solution of hydrogen peroxide applied topically initiates a chemical reaction, presumably with tissue enzymes producing oxygen, which distends lymphatic channels. Though the reaction is not specific, lymphatics have a characteristic morphology by which they are identified. After application of peroxide, clearing by a modified Spalteholz technique after injection has been utilized also. Cardiac
Johnson RA, Blake TM. Lymphatics of the Heart.. Ann Intern Med. ;62:1071–1072. doi: 10.7326/0003-4819-62-5-1071_3
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Published: Ann Intern Med. 1965;62(5):1071-1072.
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Print ISSN: 0003-4819 | Online ISSN: 1539-3704
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