Leonard Laster, M.D. (Associate); Robert K. Ockner, M.D.; Minnie L. Woodson, B.S.
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In vivo studies have shown bile salts influence not only intestinal absorption of sterols but also intestinal synthesis of sterols. We assayed scrapings of guinea pig small-intestine mucosa in vitro for incorporation of isotope from 14C-precursors into C27-sterols. Activity was greater in distal gut; animals varied markedly (1965). To test bile salt effects in vitro, we studied incorporation of acetate-2-14C or mevalonate-2-14C into C27 sterols by paired mucosal scrapings from proximal or distal small intestine of a given animal. One flask contained no bile salt, the other either mixed bile salts (TC-GC) or purified taurocholate (TC) or glycocholate (GC).
Incorporation
Laster L, Ockner RK, Woodson ML. Bile Salt Inhibition of Sterol Biosynthesis by Small-intestine Mucosa in Vitro.. Ann Intern Med. 1966;64:1161. doi: 10.7326/0003-4819-64-5-1161_2
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Published: Ann Intern Med. 1966;64(5):1161.
DOI: 10.7326/0003-4819-64-5-1161_2
Gastroenterology/Hepatology.