George E. Seiden, M.D., F.A.C.P.
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A sensitive method was developed for measuring cytotoxic effects produced by lymphoid cells harvested from rats with allergic encephalomyelitis. Allergic encephalomyelitis was produced by immunizing Lewis rats with 2.5 µg of Kies protein emulsified with Freund's complete adjuvant. Peritoneal cells were harvested 10 to 14 days after immunization. Harvested cells from specifically immunized rats were compared for cytopathic effect with equal numbers of control nonspecifically sensitized peritoneal cells. Cytopathic effect was measured by interacting lymphoid cells with HeLa or L cells, which had been grown in the presence of 14C thymidine and subsequently "coated" with a solution of Kies protein
Seiden GE. An in Vitro Test of Cellular Immunity in Allergic Encephalomyelitis.. Ann Intern Med. ;64:1168. doi: 10.7326/0003-4819-64-5-1168_1
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Published: Ann Intern Med. 1966;64(5):1168.
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Print ISSN: 0003-4819 | Online ISSN: 1539-3704
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