Roger M. Morrell, M.D.
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The intracellular site and mode of action of hypothalamic releasing factors for anterior pituitary hormones are unknown. Labeled (125I)-rabbit-antihuman growth hormone (HGH) was incubated with bovine anterior pituitary supernatant and shown on sucrose density gradient analysis to have bound to a specific polyribosomal (polysomal) peak. The bound radioactivity was liberated into the ribosomal monomer peak by pancreatic ribonuclease that also abolished the polysomes. Treatment with ovine hypothalamic extract containing growth-hormone releasing factor (SRF) resulted in a shift of radioactivity into a different (soluble) portion of the gradient. The latter treatment left the polysomes intact, indicating minimal contamination with pancreatic ribonuclease.
Morrell RM. Release of Bound Antihuman Growth Hormone from Bovine Anterior Pituitary Polyribosomes by Ribonuclease and Hypothalamic Extract.. Ann Intern Med. 1969;70:1063–1064. doi: https://doi.org/10.7326/0003-4819-70-5-1063_3
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Published: Ann Intern Med. 1969;70(5):1063-1064.
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