S. Kwan Park, M.D.; Jerome I. Brody, M.D.; Herbert A. Wallace, M.D.; William S. Blakemore, M.D.
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This study determined whether lymphocyte reactivity, a particularly important component of cellular immunity, was altered in patients undergoing various forms of surgery. The experimental protocol consisted of harvesting peripheral blood lymphocytes from cell donors before operation, within 24 hr after leaving the operating room, and at determined intervals thereafter. The lymphocytes were grown in duplicate in cell culture, each vial containing autologous plasma and phytohemagglutinin as an in vitro stimulant. A 14carbon amino acid hydrolysate was added to one of the cultures, and after 72 hr the culture was terminated, the cells disrupted by ultrasonic vibration, and the radioactive, protein-containing
Park SK, Brody JI, Wallace HA, et al. Depression of Lymphocyte Immunogenesis After Surgery.. Ann Intern Med. 1969;70:1085. doi: 10.7326/0003-4819-70-5-1085_2
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Published: Ann Intern Med. 1969;70(5):1085.
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Print ISSN: 0003-4819 | Online ISSN: 1539-3704
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