A. S. Keitt, M.D.; J. M. Salhany; R. S. Eliot, M.D., F.A.C.P.
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2,3-Diphosphoglycerate (DPG) is known to influence the oxygen equilibrium of hemoglobin and has been thought to participate in the regulation of glycolysis and nucleotide catabolism by inhibiting certain red cell enzymes. To examine further these properties of DPG in the intact red cell, fresh cells (hematocrit, 10%) were incubated with inosine, pyruvate, and phosphate (IPP), 20 mM each, for 16 hr at 37 C so as to increase their content of DPG. The glycolytic metabolism of these cells was examined during prolonged incubation after removal of IPP (Method: Keitt, J Clin Invest 48:1997, 1969). Hemoglobin function was estimated by determining
Keitt AS, Salhany JM, Eliot RS. Super Cells—Metabolic and Functional Behavior of Red Cells with Extreme Elevation of 2,3-Diphosphoglycerate.. Ann Intern Med. ;74:827. doi: 10.7326/0003-4819-74-5-827_1
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Published: Ann Intern Med. 1971;74(5):827.
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