Delano Meriwether, M.D.; Stanley P. Balcerzak, M.D., F.A.C.P.
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Factors influencing autohemolysis of paroxysmal nocturnal hemoglobinuria (PNH) RBCs were studied in vitro to further characterize the cell membrane lesion and the mechanism of hemolysis in this condition. Autohemolysis of naturally occurring PNH RBCs was measured under standard conditions of 48-hour incubation, in the absence and presence of various additives. Autohemolysis of washed PNH RBCs was also determined in vitro under controlled conditions of physiologic pH, temperature, and molarity, using barbital buffer and fresh serum. Finally, autohemolytic features of naturally occurring PNH RBCs were compared with those of artificially produced PNH RBCs, that is, normal RBCs treated with 0.3 M
Meriwether D, Balcerzak SP. Autohemolysis of Paroxysmal Nocturnal Hemoglobinuria (PNH) Erythrocytes. Effects of Controlled pH, Additives, and Heated Serum.. Ann Intern Med. 1972;76:870. doi: https://doi.org/10.7326/0003-4819-76-5-870_1
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Published: Ann Intern Med. 1972;76(5):870.
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