Martin J. Smith, M.D.; Arne Slungaard
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Normal lymphocytes respond to the presence of allogenic lymphocytes in cultures with a high uptake of 3H-thymidine. We studied the interaction of chronic lymphatic leukemia (CLL) lymphocytes with normal allogenic lymphocytes, using the oneway, mixed leukocyte culture technique of Bach, with Ficoll-Hypaque gradient, to harvest lymphocytes. The lymphocytes of 11 patients with this disease were tested separately against those of 4 unrelated normal control persons. Contamination by nonmononuclear cells was uniformly less than 5%, and viability (eosin dye exclusion) was greater than 90%. In all 11 patients an impaired responsiveness was found. The mean response of CLL lymphocytes to those
Smith MJ, Slungaard A. The Impaired Responsiveness of Chronic Lymphatic Leukemia (CLL) Lymphocytes to Allogenic Lymphocytes.. Ann Intern Med. 1972;76:871. doi: https://doi.org/10.7326/0003-4819-76-5-871_2
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Published: Ann Intern Med. 1972;76(5):871.
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Print ISSN: 0003-4819 | Online ISSN: 1539-3704
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