Edward D. Harris Jr., M.D., F.A.C.P.
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Synovial lesions of rheumatoid arthritis are morphologically nonspecific, whereas the histopathology of subcutaneous nodules is relatively specific for diagnosis. Biochemical similarities between the synovial and nodule lesions of rheumatoid arthritis were sought by studying collagenase released by rheumatoid nodules in culture in vitro. One nodule, 2 x 3 cm, from the olecranon bursa of a 47-year-old woman with rheumatoid arthritis was cut into 2-x-2-cm explants and cultured at 37 °C in media without serum in 95% O2, 5% CO2 for 1 week, with and without colchicine (0.1 μg/ml) in the media. Media were harvested daily. Collagenolytic activity, assayed using 14C-labeled
Harris ED. Collagenase Production In Vitro by Tissue From a Rheumatoid Nodule.. Ann Intern Med. 1972;76:881. doi: 10.7326/0003-4819-76-5-881_1
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Published: Ann Intern Med. 1972;76(5):881.
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Print ISSN: 0003-4819 | Online ISSN: 1539-3704
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