Michael G. Rosenfeld, M.D.; John Mendelsohn, M.D.; Itamar B. Abrass, M.D.
This content is PDF only. Please click on the PDF icon to access.
The early intracellular events initiated by antigens or phytohemagglutinin (PHA) that eventuate in transformation and mitosis of human lymphocytes are unknown. The identification of a region rich in polyriboadenylic acid (poly A) in viral and eukaryotic mRNAs facilitated the isolation of lymphocyte mRNA. Uridine labeled with 3H or [3H]adenosine was added to pure human lymphocyte cultures and poly A-rich RNA isolated after 60 minutes of incubation. Addition of PHA and dibutyryl cAMP (cyclic adenosine 3',5'-monophosphate, 1O-8M) to human lymphocyte cultures resulted in an early increase in radiolabeled poly A-rich RNA, with clear stimulation (20% to 40%) seen as early as
Rosenfeld MG, Mendelsohn J, Abrass IB. Control of Transcription in the Human Lymphocyte.. Ann Intern Med. ;78:824–825. doi: 10.7326/0003-4819-78-5-824_4
Download citation file:
Published: Ann Intern Med. 1973;78(5):824-825.
Copyright © 2019 American College of Physicians. All Rights Reserved.
Print ISSN: 0003-4819 | Online ISSN: 1539-3704
Conditions of Use