J. A. KEEL, M.S.; W. R. FINNERTY, Ph.D.; JAMES C. FEELEY, Ph.D.
We obtained four bacterial isolates from patients with Legionnaires' disease and examined them for in-vitro and in-vivo fine-structure characteristics. All isolates had an outer membrane, cytoplasmic membrane, and intracellular membrane structure. Numerous intracellular inclusions were seen, particularly from in-vivo specimens, and appeared membrane-limited. Fine-structure analysis did not reveal the presence of a definitive peptidoglycan structure. Isolation, purification, and chemical analysis of Legionnaires' disease bacterium peptidoglycan established molar ratios of alanine-glutamic acid and muramic acid-glucosamine. Diaminopimelic acid was absent in the Legionnaires' disease bacterium peptidoglycan. The Kellenberger procedure for fixation appears to be the best method for the fine-structure determination of Legionnaires' disease bacteria.
KEEL JA, FINNERTY WR, FEELEY JC. Fine Structure of the Legionnaires' Disease Bacterium: In-Vitro and In-Vivo Studies of Four Isolates. Ann Intern Med. 1979;90:652–655. doi: https://doi.org/10.7326/0003-4819-90-4-652
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Published: Ann Intern Med. 1979;90(4):652-655.
Infectious Disease, Pulmonary/Critical Care.
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