MOTOHIKO OKANO, M.D., Ph.D.; GEOFFREY M. THIELE, Ph.D.; JACK R. DAVIS, M.T.; WILLIAM M. NAUSEEF, M.D.; FRANK MITROS, M.D.; DAVID T. PURTILO, M.D.
An adenovirus type-2 was isolated from peripheral blood lymphocytes and throat washings from a patient with severe chronic active Epstein-Barr virus infection. Despite the Epstein-Barr virus reactivation, attempts to establish spontaneous lymphoblastoid cell lines from peripheral blood lymphocytes and to immortalize cord lymphocytes with throat washings were unsuccessful due to a marked cytopathic effect. The supernatants from the cultures induced cytopathic effect in cultured cord lymphocytes, MRC-5 cells, A-549 cells, or Vero cells. Virus particles with adenovirus morphology were seen by electron microscopy. Using type-specific antisera, the isolate was identified as adenovirus type-2. In addition, both Epstein-Barr virus and adenovirus type-2 genomes were seen in the colonic tissues and spleen. These results suggest that the combination of Epstein-Barr virus and adenovirus type-2 may be etiologic agents in the development of chronic active Epstein-Barr virus infection in this patient.
OKANO M, THIELE GM, DAVIS JR, NAUSEEF WM, MITROS F, PURTILO DT. Adenovirus Type-2 in a Patient with Lethal Hemorrhagic Colonic Ulcers and Chronic Active Epstein-Barr Virus Infection. Ann Intern Med. ;108:693–699. doi: 10.7326/0003-4819-108-5-693
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Published: Ann Intern Med. 1988;108(5):693-699.
Gastroenterology/Hepatology, Infectious Disease.
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