ALEC E. WITTEK, M.D., C.M.; MICHAEL A. PHELAN, Ph.D.; MARTHA A. WELLS, B.S.; LUBA K. VUJCIC, M.S.; JAY S. EPSTEIN, M.D.; H. CLIFFORD LANE, M.D.; GERALD V. QUINNAN Jr., M.D.
A sensitive enzyme immunoassay was developed for detecting human immunodeficiency virus (HIV) core antigen. Assay sensitivity was 3.67 pmol/L of purified HIV core protein, and 1 or 100 in-vitro infectious units/mL of HIV in purified virus preparations or cell culture supernatants, respectively. Enzyme immunoassay sensitivity exceeded that of reverse transcriptase assay by 1000-fold. Core antigen was detected in whole plasma from 41% of symptomatic subjects and 13% of asymptomatic subjects seropositive for HIV. After plasma fractionation, antigenemia was found in 60% of symptomatic subjects and in 33% of asymptomatic subjects seropositive for HIV. Fifty-seven percent of samples from which HIV could be isolated in lymphocyte culture had detectable quantities of core antigen in plasma. However, at least 87% of samples with measurable antigen in plasma had HIV isolated from lymphocyte cultures. Antigenemia was associated with reduced T-cell number and symptomatic disease, and may be a useful marker for disease progression.
WITTEK AE, PHELAN MA, WELLS MA, et al. Detection of Human Immunodeficiency Virus Core Protein in Plasma by Enzyme Immunoassay: Association of Antigenemia with Symptomatic Disease and T-Helper Cell Depletion. Ann Intern Med. 1987;107:286–292. doi: 10.7326/0003-4819-107-2-286
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Published: Ann Intern Med. 1987;107(3):286-292.
HIV, Infectious Disease.
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